PolyETHYL A Columns

These materials separate proteins on the basis of hydrophobic character, as does Reversed-Phase Chromatography. HIC uses totally aqueous buffers, maintaining tertiary structure and biological activity. Typically, a sample is eluted with a decreasing gradient of a salt such as sulfate or phosphate. Proteins elute in order of increasing surface hydrophobicity. Surfactants (e.g. CHAPS, octylglucoside) can be added to the mobile phase if necessary. The relative hydrophobic character of PolyETHYL A is 60.

When To Use HIC:

  1. Characterization of antibodies.
  2. Purification of polypeptides such as glycopeptides and venoms.
  3. Isolation of proteins from crude extracts.
  4. Quality control assay using a method complementary to ion-exchange and Reversed-Phase.
  5. Isolation of integral membrane proteins and their complexes.

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Item #

Item Name

Particle Size

Pore Size

Column Inside Diameter

Column Length

List Price

051ET0503

PolyEthyl A 5um 300A 1.0 x 50m

5 µm

300 A

1 mm

50 mm

$437.75
151ET0503

PolyEthyl A 5um 300A 1 x 150mm

5 µm

300 A

1 mm

150 mm

$592.25
202ET0503

PolyETHYL A column 200x2.1mm (

5 µm

300 A

2.1 mm

200 mm

$556.20
3.54ET0503

PolyEthyl A 5um 300A 4.6 x 35m

5 µm

300 A

4.6 mm

35 mm

$370.80
104ET0503

PolyETHYL A column 100x4.6mm (

5 µm

300 A

4.6 mm

100 mm

$468.65
204ET0503

PolyETHYL A column 200x4.6mm (

5 µm

300 A

4.6 mm

200 mm

$556.20
209ET0503

PolyETHYL A column 200x9.4mm (

5 µm

300 A

9.4 mm

200 mm

$1,091.80
2521ET0503

PolyEthyl A 5um 300A 21 x 250m

5 µm

300 A

21 mm

250 mm

$3,296.00
051ET0510

PolyEthyl A 5um 1000A 1.0 x 50

5 µm

1000 A

1 mm

50 mm

$437.75
151ET0510

PolyEthyl A 5um 1000A 1 x 150m

5 µm

1000 A

1 mm

150 mm

$592.25
202ET0510

PolyETHYL A column 200x2.1mm (

5 µm

1000 A

2.1 mm

200 mm

$556.20
3.54ET0510

PolyEthyl A 5um 1000A 4.6 x 35

5 µm

1000 A

4.6 mm

35 mm

$370.80
104ET0510

PolyETHYL A column 100x4.6mm (

5 µm

1000 A

4.6 mm

100 mm

$468.65
204ET0510

PolyETHYL A column 200x4.6mm (

5 µm

1000 A

4.6 mm

200 mm

$556.20
209ET0510

PolyEthyl A 5um 1000A 9.4 x 20

5 µm

1000 A

9.4 mm

200 mm

$1,019.70
2521ET0510

PolyEthyl A 5um 1000A 21 x 250

5 µm

1000 A

21 mm

250 mm

$3,296.00
204ET1203

PolyEthyl A 12um 300A 4.6 x 20

12 µm

1000 A

4.6 mm

200 mm

$324.45
209ET1203

PolyETHYL A column 200x9.4mm (

12 µm

1000 A

9.4 mm

200 mm

$782.80
204ET1210

PolyEthyl A 12um 1000A 4 6 x 2

12 µm

1000 A

4.6 mm

200 mm

$324.45
209ET1210

PolyEthyl A 12um 1000A 9.4 x 2

12 µm

1000 A

9.4 mm

200 mm

$762.20
051ET05006

PolyEthyl A 5um 60A 1.0 x 50mm

5 µm

60 A

1 mm

50 mm

$437.75
051ET0502

PolyEthyl A 5um 200A 1.0 x 50m

5 µm

200 A

1 mm

50 mm

$437.75
051ET0505

PolyEthyl A 5um 500A 1.0 x 50m

5 µm

500 A

1 mm

50 mm

$437.75
054ET0510

PolyETHYL A column 50x4.6mm (5

5 µm

1000 A

4.6 mm

50 mm

$406.85
104ET05006

PolyEthyl A 5um 60A 4.6 x 100m

5 µm

60 A

4.6 mm

100 mm

$442.90